Our research group is mainly involved in the studies of the metabolism and physiological functions of growth regulators, polyamines and phenolic compounds in plants. We investigate the role of these biologically active compounds in plant development and in the response of plants to abiotic stresses.

In our experiments we use the diverse plant systems from the whole plants to the cell cultures. Our research is primarily focused on somatic embryogenesis of conifers. In the scope of this theme we study the regulation of somatic embryo development, the role of phytohormones in somatic embryogenesis and the effects of abiotic stresses on somatic embryos.

We use a wide array of approaches:

  • Microscopy – light, confocal and electron microscopy, enhanced by advanced computer image analysis

  • Biochemical methods – studies of activities of enzymes involved in metabolism of biologically active compounds (e.g. radiometry)

  • Molecular biology methods – specific gene expressions, and transformation of tissue cultures

  • Analytical methods – qualitative and quantitative determination of biologically active compounds by gas- and liquid chromatography in tandem with mass spectroscopic detection (cooperation with the IEB Laboratory of mass spectrometry).

The effect of storage on the profile of phenolic compounds in selected apple varieties

Submitted by Kateřina Eliášová on Fri, 08/03/2018 - 10:50

Apples are considered as one of the most important fruit crop with excellent health benefits and extensive
area of cultivation. Majority of their benefits is associated with a relatively high content of antioxidants
including phenolic compounds that belong to the health-promoting phytochemicals.
Phenolic compounds constitute a substantial and an important group of phenylpropanoids produced by
plants as secondary metabolites. The health-promoting effects of phenolic compounds depend on their

The biochemical characteristics of the physiological activity of beech and spruce embryos

Submitted by Kateřina Eliášová on Tue, 03/27/2018 - 12:03

It is necessary to find convenient and measurable criteria for the evaluation of seeds/embryos quality before germination because of a long time interval between the preparation of seeds or embryos (before germination) and obtaining the seedlings or emblings. The success of all treatments before germination is assessed by the quality of the newly formed plants only.

Unbiased estimation of the proportion of non-embryogenic cell clusters in the somatic embryogenic culture of Douglas-fir

Submitted by Kateřina Eliášová on Mon, 02/12/2018 - 23:26

Embryogenic cultures of Douglas-fir were induced from immature zygotic embryos. They proliferate as embryonal masses, however, in some lines of Douglas-fir, non-embryogenic cell (NECs) clusters interspersed with early somatic embryos (SEs) were observed. In order to evaluate the differences between lines we wanted to quantify the proportion of the SEs and NECs in the embryonal mass. For estimation of this proportion (volume density) we used stereological point-counting method based on counting points of the test grid falling in the tissue under study.