Research

Biochemical and morphological parameters determinig cryotolerance in conifer embryogenic cultures.

Annotation: 

The aim of the COST Action CA15124 - A new Network of European BioImage Analysts to advance life science imaging (NEUBIAS) is to maximise the outputs of image-based research in life sciences by empowering the international network of bioimage analysis (IA) experts - BIAlysts.

Cultivation and genetic factors affecting efficiency of conifer somatic embryogenesis.

Cultivation and genetic factors affecting efficiency of conifer somatic embryogenesis.

MŠMT, 2000-2004, Martin Vágner

• National Institute for Agricultural Research (INRA), Unit for Improvement, Genetics and Forestry Physiology (UR 0588), Orléans, France
• Institute of Plant Genetics and Biotechnology of the Slovak Academy of Sciences, Nitra, Slovakia
• Department of Experimental Plant Biology, Faculty of Science, Charles University, Praha, Czech Republic
• Institute of Scientific Instruments of the Czech Academy of Sciences, Brno, Czech Republic
• Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences, Praha, Czech Republic
• Department of Forest Ecology, Faculty of Forestry and Wood Science, Czech University of Life Sciences, Praha, Czech Republic

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The aim of the project was to evaluate the alterations in polyamine metabolism and in the levels of hydroxycinnamic acids, flavonoids and stilbenes in the stress response of somatic embryos of Norway spruce to drought and high temperature. The determined contents of abscisic acid and auxin contributed to understanding the abscisic acid-auxin cross-talk in the response to stress. The extent of lipid peroxidation (as indicated by MDA levels) in stressed somatic embryos was also investigated. Histochemical tests for the presence of polyphenolic substances on embryo sections after the exposure of somatic embryos to UV-B completed the biochemical analyses.

Annotation:

The aim of the project was to evaluate the alterations in polyamine biosynthetic enzymes and polyamine contents and the levels of hydroxycinnamic acids and flavonoids after the exposure of embryogenic tissue cultures of Norway spruce to UV-B radiation. Histochemical tests for the presence of polyphenolic substances (with UV-B protecting character) on embryo sections completed the biochemical analyses.

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The aim of the project was to elucidate the role of proline and polyamines, metabolites with the crucial protective functions, in the stress response of tobacco plants to high temperature and drought.

Characterization of defence response in wild plants and in transgenic plants with modified P5CS gene (P5CSF129A), which codes for the key enzyme of proline biosynthesis, contributed to understanding of the function of these protective elements in plant abiotic stress tolerance.

• Hebrew University, Institute for Drug Research, Jerusalem, Israel 
• Forestry and Game Management Research Institute, Strnady, Czech Republic
• Forestry and Game Management Research Institute, Department of Reproductive Sources, Research station Kunovice, Czech Republic

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The overall goal of the project is to determine the factors responsible for the inefficiently of existing, pre-sowing treatments so that beechnut germination and subsequent emergence of beech germinants can be improved. The results will be used to develop protocols for providing better and more economical use of beechnuts including field emergence and for obtaining sufficient quantities of seedlings for reforestation.