What is the best way to maintain embryogenic capacity of embryogenic lines initiated from Douglas-fir immature embryos?
Douglas-fir (Pseudotsuga menziesii (Mirb) Franco) is a native conifer from the Pacific North-West of the US and Canada, and is one of the most important timber species used in the world. Douglas-fir is highly productive in a range of climatic conditions and has valuable wood properties (quality) as well as a strong tolerance to diseases and insects. In Europe, Douglas-fir is a major species for reforestation with increasing demand for its wood. Adaptation of new varieties to climate change and associated stresses is one challenging question for ongoing breeding programs.