Our research group is mainly involved in the studies of the metabolism and physiological functions of growth regulators, polyamines and phenolic compounds in plants. We investigate the role of these biologically active compounds in plant development and in the response of plants to abiotic stresses.
In our experiments we use the diverse plant systems from the whole plants to the cell cultures. Our research is primarily focused on somatic embryogenesis of conifers. In the scope of this theme we study the regulation of somatic embryo development, the role of phytohormones in somatic embryogenesis and the effects of abiotic stresses on somatic embryos.
We use a wide array of approaches:
Microscopy – light, confocal and electron microscopy, enhanced by advanced computer image analysis
Biochemical methods – studies of activities of enzymes involved in metabolism of biologically active compounds (e.g. radiometry)
Molecular biology methods – specific gene expressions, and transformation of tissue cultures
Analytical methods – qualitative and quantitative determination of biologically active compounds by gas- and liquid chromatography in tandem with mass spectroscopic detection (cooperation with the IEB Laboratory of mass spectrometry).