In vitro transformation of hemp and cannabis by Agrobacterium tumefaciens. Hypocotyl segments of technical and young leaves of medical cannabis were used as source material.
Organogenesis in vitro – cotyledon segments from industrial hemp (Cannabis sativa subsp. sativa L.) seedlings (cultivars Felina32, Finola, Futura75, Orion33, Santhica27 , USO31).
Calli of different types, buds and roots are formed not only on the cutting surface.
New cells growing on the former ZE either form calluses of different patterns and colours or differentiate to suspensor and meristem cells of early somatic embryos founding new embryogennic culture.
New somatic embryogennic cultures can be induced from zygotic embryos cultivated in vitro on the induction medium. Phytohormones auxins and cytokinins play important role in this phase.
Somatic embryogenic culture can be induced from immature zygotic embryos extirped from seeds in the course of summer.