Tags: phytohormones

The transcriptomic (RNA-Sequencing) datasets collected in the course of floral induction in Chenopodium ficifolium 459

Submitted by Kateřina Eliášová on Wed, 07/20/2022 - 14:09

The transition from vegetative growth to reproduction is an essential commitment in plant life. It is triggered by environmental cues (day length, temperature, nutrients) and regulated by the very complex signaling gene network and by phytohormones. The control of flowering is well understood in Arabidopsis thaliana and in some crops, much less is known about the other angiosperms.

The high concentrations of abscisic, jasmonic, and salicylic acids produced under long days do not accelerate flowering in Chenopodium ficifolium 459

Submitted by Kateřina Eliášová on Fri, 04/08/2022 - 11:59

The survival and adaptation of angiosperms depend on the proper timing of flowering. The weedy species Chenopodium ficifolium serves as a useful diploid model for comparing the transition to flowering with the important tetraploid crop Chenopodium quinoa due to the close phylogenetic relationship. The detailed transcriptomic and hormonomic study of the floral induction was performed in the short-day accession C. ficifolium 459. The plants grew more rapidly under long days but flowered later than under short days.

Dormancy breaking in Fagus sylvatica seeds is linked to formation of abscisic acid-glucosyl ester

Submitted by Kateřina Eliášová on Thu, 09/26/2019 - 13:20

Seed dormancy is an adaptive mechanism that allows seed germination under suitable environmental conditions. Germination of stored dormant seeds proceeds after dormancy breaking induced by stratification. To improve understanding of dormancy breaking in beechnuts, we: investigated effects of moisture content and temperature during storage; analysed contents of abscisic acid, abscisic acid metabolites and indole-3-acetic acid in embryonic axes during storage and stratification; and histochemically localized storage proteins.

Profiles of endogenous phytohormones over the course of Norway spruce somatic embryogenesis

Submitted by Kateřina Eliášová on Tue, 08/21/2018 - 13:24

Conifer somatic embryogenesis (SE) is a process driven by exogenously supplied plant growth regulators (PGRs). Exogenous PGRs and endogenous phytohormones trigger particular ontogenetic events. Complex mechanisms involving a number of endogenous phytohormones control the differentiation of cells and tissues, as well as the establishment of structures and organs. Most of the mechanisms and hormonal functions in the SE of conifers have not yet been described.

Approaches we apply to study Norway spruce somatic embryogenesis

Submitted by Lucie Fischerová on Tue, 05/30/2023 - 11:31

Our expertise cover methods of anatomy, histochemical detections, and indirect immunofluorescence, determination of the content of plant hormones, and analysis of gene expression. Newly we are also implementing methods of in-vitro virus detection. To control the developmental processes of in-vitro cultures, we use a broad spectrum of newly synthesized bioactive molecules as well as modulators of plant hormone metabolism and perception (e.g. anti-auxins, anti-gibberellins, cytokinin derivatives).

Physiology and role of plant growth regulators in somatic embryogenesis

Submitted by Zuzana Vondráková on Tue, 02/13/2018 - 09:08

Vondráková Z, Krajňáková J, Fischerová L, Vágner M, Eliášová K:

Physiology and role of plant growth regulators in somatic embryogenesis. 

In: Vegetative Propagation of Forest Trees, ed. Park YS, Bonga JM, Moon HK, National Institute of Forest Trees, Seoul, Korea, pp. 123-169, 2016.

Endogenous phytohormone profiles during Norway spruce somatic embryogenesis

Submitted by Zuzana Vondráková on Mon, 02/12/2018 - 23:13

Somatic embryogenesis (SE) in conifers is governed by a complex network of hormonal metabolic and signaling pathways. Changes in the patterns and concentrations of endogenous phytohormones including auxins, cytokinins (CKs), abscisic acid (ABA), jasmonates, and salicylic acid (SA) were analyzed in the course of SE in Norway spruce (Picea abies).