Tags: autophagy

Wholemount immunolabeling and stereological quantification of autophagosomes in Arabidopsis thaliana root epidermal cells

Submitted by Kateřina Eliášová on Wed, 03/06/2024 - 16:03

The workflow enables the quantification of autophagosomes in Arabidopsis thaliana root epidermal cells in 3D. It combines immunolabeling of an autophagosome marker ATG8 with commercially available anti-ATG antibody and the subsequent stereological quantification of the immunolabeled particles. The immunolabeled samples are imaged with a confocal microscope and Z-stacks are acquired.

Internal authors

A novel workflow for unbiased quantification of autophagosomes in 3D in Arabidopsis thaliana roots

Submitted by Kateřina Eliášová on Wed, 03/06/2024 - 15:46

Macroautophagy is often quantified by live imaging of autophagosomes labeled with fluorescently tagged ATG8 protein (FP-ATG8) in Arabidopsis thaliana. The labeled particles are then counted in single focal planes. This approach may lead to inaccurate results as the actual 3D distribution of autophagosomes is not taken into account and appropriate sampling in the Z-direction is not performed.

Internal authors

Assessment of autophagy in plant cells

Submitted by Kateřina Eliášová on Tue, 04/21/2020 - 14:26

Autophagy is an evolutionary conserved catabolic process deemed to maintain or restore cellular and organismal homeostasis. In plants, basal autophagy is essential for growth and development, it is required for nutrient remobilization during senescence and nutrient deficiency, for removal of organelles and macromolecules formed during plant development or damaged by environmental stresses.

Polyamine metabolism after induction of autophagy in tobacco BY2 cell culture

Submitted by Kateřina Eliášová on Thu, 10/13/2022 - 11:37

Polyamines putrescine (Put), spermidine (Spd), and spermine (Spm) are ubiquitous, small aliphatic polycations found in eukaryotic organisms, which regulate vital developmental and physiological events. They play an important role in diverse plant growth and developmental processes and adaptation to environmental stresses. Among other functions, spermidine stimulates the process of autophagy across species including yeast, animals, and even humans.

Polyamine metabolism and autophagy in plants

Submitted by Kateřina Eliášová on Thu, 10/13/2022 - 11:16

Polyamines putrescine (Put), spermidine (Spd), and spermine (Spm) are ubiquitous, small aliphatic polycations found in eukaryotic organisms, which regulate vital developmental and physiological events. They play an important role in diverse plant growth and developmental processes and adaptation to environmental stresses. Among other functions, spermidine stimulates the process of autophagy across species including yeast, animals, and even humans.