Tags: chitinases

The humidity level matters during the desiccation of Norway spruce somatic embryos

Submitted by Kateřina Eliášová on Fri, 07/29/2022 - 09:57

In Norway spruce, as in many other conifers, the germination capacity of somatic embryos is strongly influenced by the desiccation phase inserted after maturation. The intensity of drying during desiccation eminently affected the formation of emblings (i.e., seedlings developed from somatic embryos). Compared to non-desiccated embryos, the germination capacity of embryos desiccated at 100% relative humidity was about three times higher, but the reduction of relative humidity to 95% and 90% had a negative effect on the subsequent embryo development.

The effect of different air humidity during desiccation on the development of Norway spruce somatic embryos

Submitted by Lucie Fischerová on Fri, 09/21/2018 - 12:38

The objective of the study was to follow morphological, selected biochemical and transcriptional characteristics induced by various air humidity during desiccation of Norway spruce somatic embryos. The level of free polyamines lowered, higher forms of polyamines were favoured. Expression profiles of monitored genes were variously influenced by different relative air humidity. Our data proved, that desiccation of somatic embryos is metabolically active process highly affected by relative air humidity.

The effect of drought stress on Norway spruce somatic embryo development

Submitted by Kateřina Eliášová on Fri, 08/03/2018 - 11:51

Somatic embryogenesis is a developmental process where a plant somatic cell dedifferentiate to a totipotent embryonic stem cell that has the ability to give rise to an embryo under appropriate conditions. Desiccation is the final phase of normal embryonic development in most angiosperms and appears to be important in the transition from embryogeny to the ability to germinate and form normal seedlings.

Beta-1,3-glucanases and chitinases in somatic embryos of Norway spruce

Submitted by Lucie Fischerová on Mon, 02/12/2018 - 23:31

Somatic embryogenesis is a developmental process by which a plant somatic cell can dedifferentiate to a totipotent embryonic stem cell that has the ability to give rise to an embryo under appropriate conditions. Desiccation is the final phase of normal embryonic development in most angiosperms and appears to be important in the transition from embryogeny to the ability to germinate and form normal seedlings. Different conditions during desiccation affect the biochemical changes that occur during this phase.