Assessment of somaclonal variation in indirect morphogenesis-derived plants of Arracacia xanthorrhiza | Laboratory of Biologically Active Compounds

Callus induction and plant regeneration of A. xanthorrhiza. (a) Callus with meristematic centres (bar = 3 mm) (b) Histological analysis of morphogenic callus (MC: Meristematic Centers) (bar = 200 µm ) (c) Shoots regeneration from callus (bar = 4 mm) (d) Germinated somatic embryo (bar = 1.2 mm) (e) Histological analysis of early cotyledonary somatic embryo (Ct: Cotyledon; Hy: Hypocotyl; Ra: Radicle; SA: Shoot Apex) (bar = 500 µm) (f) Plant recovered from callus (bar = 5 mm)

The objective of this work was to induce and detect somaclonal variation in arracacha (Arracacia xanthorrhiza) plants regenerated via indirect morphogenesis, in order to evaluate the potential of this technique to produce new genotypes for breeding purposes of this crop.