|Title||Menu category||Text||Tags||Author||Year ended|
|Somatic embryogenesis of conifers||Research Topics||
The process of plant somatic embryogenesis allows the acquisition of a large number of genetically identical individuals. That fact is used in practice to obtain a rich progeny from a minimal number of seeds. At the same time, somatic embryogenesis is an ideal system for studying the early stages of plant development. Somatic embryogenesis starts by the derivation of embryogenic culture, mostly from zygotic embryos. Embryogenic cultures are cultivated in vitro on precisely defined media, whose composition determines further development of somatic embryos. In conifers the whole process consists of five basic steps - induction of embryogenic culture, proliferation, maturation of somatic embryos, desiccation and germination.
|Picea abies, somatic embryogenesis||Kateřina Eliášová|
|Metabolism of polyamines||Research Topics||
Polyamines (PAs), low molecular mass polycations, are ubiquitous cell components that are essential for normal growth of both eukaryotic and prokaryotic cells. Most of the biological functions of PAs can be explained by their polycationic nature, which facilitates interactions with anionic macromolecules (such as DNA and RNA) and negatively charged groups of membranes. They are implicated in a wide range of biological processes including cell division and growth, morphogenesis and differentiation and responses to biotic and abiotic stresses. The most abundant PAs in plants are putrescine (Put), spermidine (Spd) and spermine (Spm).
|polyamines, putrescine, spermidine, spermine||Lenka Gemperlová|
|The use of biotechnology methods for the preservation and reproduction of autochtonous populations of hurst ecotype of Norway spruces||Past Projects||
The obtained results contributed to understanding of the role of polyamines and additional biological mechanisms in optimization of the induction of embryogenic lines and somatic embryo conversion of Hurst ecotype of Norway spruce. New knowledge on the genetic diversity of Hurst ecotype of Norway spruce will be used in reproduction the required genotypes.
|The profile of carotenoids in selected apple varieties in relation to storage conditions||Past Projects||
We extend the information dealt with the concentration and composition of antioxidants in selected apple varieties from IEB. The research activity of the Station of apple breeding is focused on research of resistance against diseases in apples and on breeding of scab resistant and powdery mildew tolerant apple varieties. Breeding for resistance to these diseases has a high priority throughout the world in view of minimizing the use of chemicals in fruit production.
|Biochemical and morphological parameters determinig cryotolerance in conifer embryogenic cultures||Past Projects||
Biochemical and morphological parameters determinig cryotolerance in conifer embryogenic cultures.
|The role of polyamines in the process of plant autophagy||Past Projects||
The aim of the project is to evaluate the relationship between polyamine metabolism and autophagy in plants. Autophagy will be followed in two experimental systems - in tobacco BY-2 cell culture and in coniferous embryogenic cultures. Firstly, the polyamine metabolism will be monitored during induced autophagy, then the process of autophagy will be studied after exogenous application of higher polyamines or endogenous modulation of polyamine synthesis. The obtained results will contribute to understanding of the function of polyamines in plant autophagy.
|Role of ABSCISIC ACID.INSENSITIVE3 homologue in abscisic acid signal transduction studied in system of Norway spruce somatic embryogenesis||Past Projects||
Role of ABSCISIC ACID.INSENSITIVE3 homologue in abscisic acid signal transduction studied in system of Norway spruce somatic embryogenesis.
|Contribution of super-resolution microscopy and image analysis to the study of plant in vitro cultures with the emphasis on somatic embryogenesis of conifers||Past Projects||
The aim of the COST Action CA15124 - A new Network of European BioImage Analysts to advance life science imaging (NEUBIAS) is to maximise the outputs of image-based research in life sciences by empowering the international network of bioimage analysis (IA) experts - BIAlysts.