MŠMT, 2024-2026, Lucie Fischerová
Annotation
The aim of the project is to test the effect of topolin-type cytokinins (CK) as an alternative to N6-benzyladenine (BA) in the induction and proliferation of Norway spruce embryogenic cultures. BA and its degradation products are very stable. From many other in vitro plant propagation systems, the negative effect of 9-glucoside is known, inhibiting both growth and rooting of the plants produced. CKs will be applied during the induction stage to overcome genotype-dependent recalcitrance, and also during the proliferation to test if they can increase the quantity and quality of produced somatic embryos and subsequently their germination.
Date ended