Periodika: JOURNAL OF EXPERIMENTAL BOTANY

A novel workflow for unbiased quantification of autophagosomes in 3D in Arabidopsis thaliana roots

Submitted by Kateřina Eliášová on Wed, 03/06/2024 - 15:46

Macroautophagy is often quantified by live imaging of autophagosomes labeled with fluorescently tagged ATG8 protein (FP-ATG8) in Arabidopsis thaliana. The labeled particles are then counted in single focal planes. This approach may lead to inaccurate results as the actual 3D distribution of autophagosomes is not taken into account and appropriate sampling in the Z-direction is not performed.

Tags
autophagy
stereology
Arabidopsis thaliana
root
immunolabelling
Internal authors
Kateřina Eliášová

Diurnal changes in polyamine content, arginine and ornithine decarboxylase, and diamine oxidase in tobacco leaves

Submitted by Lenka Gemperlová on Tue, 02/13/2018 - 13:20

Gemperlová L, Nováková M, Vaňková R, Eder J, Cvikrová M:

Diurnal changes in polyamine content, arginine and ornithine decarboxylase, and diamine oxidase in tobacco leaves.

JOURNAL OF EXPERIMENTAL BOTANY 57: 1413-1421, 2006.

Internal authors
Lenka Gemperlová
Josef Eder
Milena Cvikrová

A darklight transition triggers expression of the floral promoter CrFTL1 and downregulates CONSTANS-like genes in a short-day plant Chenopodium.

Submitted by Jan Kolář on Tue, 02/13/2018 - 09:46

Drabešová J, Cháb D, Kolář J, Haškovcová K, Štorchová H:

A darklight transition triggers expression of the floral promoter CrFTL1 and downregulates CONSTANS-like genes in a short-day plant Chenopodium.

JOURNAL OF EXPERIMENTAL BOTANY 65: 2137-2146, 2014.

Internal authors
Jan Kolář