Externí autoři: Vilém Neděla

The response of Picea abies somatic embryos to UV-B radiation depends on the phase of maturation

Submitted by Kateřina Eliášová on Tue, 11/27/2018 - 11:17

In summary, the exposure of Norway spruce EC to UV-B radiation at different stages of maturation had the strongest effect on cell viability of embryos at early stages of development and resulted in the inhibition of somatic embryo development and/or the substantial decrease in the number of embryos. The extent of cell damage was dependent on the UV-B dose applied, as well as the embryo developmental stage, and might be related to differentiation of the outermost cell layers and formation of protoderm.

The development of Norway spruce somatic embryos visualized with different microscopic tools

Submitted by Kateřina Eliášová on Mon, 02/12/2018 - 23:22

Somatic embryogenesis represents a useful system for the study of the process of embryogenesis as well as a convenient system for the clonal propagation of conifers. It consists of 5 steps: induction of embryogenic culture from somatic cells of zygotic embryos; proliferation, i.e. the development and multiplication of early somatic embryos; maturation, desiccation, and germination followed by embling´s development.