Assessment of somaclonal variation in somatic embryo-derived plants of yacon [Smallanthus sonchifolius (Poepp. and Endl.) H. Robinson] using inter simple sequence repeat analysis and flow cytometry

Fig. 1. Somatic embryogenesis in yacon. (a) Callus (black arrow: non-embryogenic callus, white arrow: proembryogenic callus) (bar, 2 mm). (b) Proembryonic callus (bar, 1.5 mm). (c) Globular to early heart stage embryos (bar, 1 mm). (d) Embryo in early-cotyledonary stage (bar, 4 mm). (e) Cotyledonary embryo with a ruptures (bar, 0.2 mm). (f) Formation of adventitious roots in abnormal embryos (bar, 0.4 mm). (g) Germinated embryo (bar, 0.4 mm). (h) Plantlet regenerated (bar, 1.2 cm).

Yacon (Smallanthus sonchifolius) is a root crop native to the Andean region. Low sexual reproductive capacity is a major constraint facing the genetic breeding of this crop. Biotechnological techniques offer alternative ways to widen genetic variability. We investigated somaclonal variation in regenerants of yacon derived from in vitro somatic embryogenesis using simple sequence repeat (ISSR) analysis and flow cytometry.

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