Typy publikací: Časopis

The impact of the application of compochar on soil moisture, stress, yield and nutritional properties of legumes under drought stress

Napsal uživatel Kateřina Eliášová dne St, 06.03.2024 - 16:15

Nowadays, when climate change is becoming more and more evident, drought stress plays a very important role, including in agriculture. The increasing number of years with extreme temperatures in the Czech Republic has a negative impact on agricultural production, among other things. Therefore, ways are being sought to reduce these negative impacts. One of them may be the use of compochar (a mixture of compost and biochar) to improve water retention in the soil.

Interní autoři

Wholemount immunolabeling and stereological quantification of autophagosomes in Arabidopsis thaliana root epidermal cells

Napsal uživatel Kateřina Eliášová dne St, 06.03.2024 - 16:03

The workflow enables the quantification of autophagosomes in Arabidopsis thaliana root epidermal cells in 3D. It combines immunolabeling of an autophagosome marker ATG8 with commercially available anti-ATG antibody and the subsequent stereological quantification of the immunolabeled particles. The immunolabeled samples are imaged with a confocal microscope and Z-stacks are acquired.

Interní autoři

A novel workflow for unbiased quantification of autophagosomes in 3D in Arabidopsis thaliana roots

Napsal uživatel Kateřina Eliášová dne St, 06.03.2024 - 15:46

Macroautophagy is often quantified by live imaging of autophagosomes labeled with fluorescently tagged ATG8 protein (FP-ATG8) in Arabidopsis thaliana. The labeled particles are then counted in single focal planes. This approach may lead to inaccurate results as the actual 3D distribution of autophagosomes is not taken into account and appropriate sampling in the Z-direction is not performed.

Interní autoři

The FLOWERING LOCUS T LIKE 2-1 gene of Chenopodium triggers precocious flowering in Arabidopsis seedlings

Napsal uživatel Kateřina Eliášová dne Po, 31.07.2023 - 11:40

The FLOWERING LOCUS T like 2–1 gene of Chenopodium ficifolium and Chenopodium quinoa acts as a strong activator of flowering in Arabidopsis, triggering flowering at cotyledon stage and causing lethality when overexpressed.

The humidity level matters during the desiccation of Norway spruce somatic embryos

Napsal uživatel Kateřina Eliášová dne Pá, 29.07.2022 - 09:57

In Norway spruce, as in many other conifers, the germination capacity of somatic embryos is strongly influenced by the desiccation phase inserted after maturation. The intensity of drying during desiccation eminently affected the formation of emblings (i.e., seedlings developed from somatic embryos). Compared to non-desiccated embryos, the germination capacity of embryos desiccated at 100% relative humidity was about three times higher, but the reduction of relative humidity to 95% and 90% had a negative effect on the subsequent embryo development.

The transcriptomic (RNA-Sequencing) datasets collected in the course of floral induction in Chenopodium ficifolium 459

Napsal uživatel Kateřina Eliášová dne St, 20.07.2022 - 14:09

The transition from vegetative growth to reproduction is an essential commitment in plant life. It is triggered by environmental cues (day length, temperature, nutrients) and regulated by the very complex signaling gene network and by phytohormones. The control of flowering is well understood in Arabidopsis thaliana and in some crops, much less is known about the other angiosperms.

The high concentrations of abscisic, jasmonic, and salicylic acids produced under long days do not accelerate flowering in Chenopodium ficifolium 459

Napsal uživatel Kateřina Eliášová dne Pá, 08.04.2022 - 11:59

The survival and adaptation of angiosperms depend on the proper timing of flowering. The weedy species Chenopodium ficifolium serves as a useful diploid model for comparing the transition to flowering with the important tetraploid crop Chenopodium quinoa due to the close phylogenetic relationship. The detailed transcriptomic and hormonomic study of the floral induction was performed in the short-day accession C. ficifolium 459. The plants grew more rapidly under long days but flowered later than under short days.

Desiccation as a post-maturation treatment helps complete maturation of Norway spruce somatic embryos: carbohydrates, phytohormones and proteomic status

Napsal uživatel Kateřina Eliášová dne Po, 14.02.2022 - 11:30

Exposure of Norway spruce (Picea abies) somatic embryos and those of many other conifers to post-maturation desiccation treatment significantly improves their germination. An integration analysis was conducted to understand the underlying processes induced during the desiccation phase at the molecular level. Carbohydrate, protein and phytohormone assays associated with histological and proteomic studies were performed for the evaluation of markers and actors in this phase.

Micropropagation of an ornamental shrub Disanthus cercidifolius Maxim. and using ISSR and flow cytometry

Napsal uživatel Kateřina Eliášová dne Út, 05.01.2021 - 11:57

This study aimed to develop an efficient micropropagation protocol for Disanthus cercidifolius Maxim., an ornamental shrub. Sprouting buds of two genotypes (‘Trubaʼ and ‘PdSʼ) were used as an initial plant material. 

Interní autoři

The effect of storage conditions on the carotenoid and phenolic acid contents of selected apple cultivars

Napsal uživatel Kateřina Eliášová dne Po, 20.07.2020 - 15:53

The present study investigated how different storage conditions affect antioxidant levels in three scab-resistant and powdery mildew-tolerant apple cultivars. The contents of carotenoids, along with free and glycosylated phenolic acids, were quantified in harvested apples and apples stored in boxes with controlled atmospheric conditions (1.2% O2 and 2.2% CO2) and temperature (1 °C), boxes with regulated temperature (1 °C) and storage rooms with a temperature between 1 and 4 °C.