Typy publikací: Konference - poster

Our expertise cover methods of anatomy, histochemical detections, and indirect immunofluorescence, determination of the content of plant hormones, and analysis of gene expression. Newly we are also implementing methods of in-vitro virus detection. To control the developmental processes of in-vitro cultures, we use a broad spectrum of newly synthesized bioactive molecules as well as modulators of plant hormone metabolism and perception (e.g. anti-auxins, anti-gibberellins, cytokinin derivatives).

Polyamines putrescine (Put), spermidine (Spd), and spermine (Spm) are ubiquitous, small aliphatic polycations found in eukaryotic organisms, which regulate vital developmental and physiological events. They play an important role in diverse plant growth and developmental processes and adaptation to environmental stresses. Among other functions, spermidine stimulates the process of autophagy across species including yeast, animals, and even humans.

Polyamines putrescine (Put), spermidine (Spd), and spermine (Spm) are ubiquitous, small aliphatic polycations found in eukaryotic organisms, which regulate vital developmental and physiological events. They play an important role in diverse plant growth and developmental processes and adaptation to environmental stresses. Among other functions, spermidine stimulates the process of autophagy across species including yeast, animals, and even humans.

Under standard cultivation conditions, Norway spruce somatic embryos (SEs) accumulate polyphenolic compounds mostly in the root cap. In response to UV-B, the accumulation of polyphenolics increases in protodermal cells and subprotodermal cortical cells and idioblasts in hypocotyl and cotyledons where they can attenuate UV-B stress. To quantify polyphenolic compounds accumulated in somatic embryos on the histological level resin sections were prepared, stained with Toluidine Blue.

The aim of this work was to determine the concentration of selected carotenoids (neoxanthin, violaxanthin, antheraxanthin, lutein, zeaxanthin and ß-carotene) and phenolic acids in selected scab resistant and powdery mildew tolerant apple varieties originating from the Station of apple breeding of the IEB. We investigated three different apple varieties - Luna (yellow), Red Topaz (red) and Karneval (streaked) for their antioxidant contents in peel and flesh immediately after the harvest and after 7 months of storage.

The objective of the study was to follow morphological, selected biochemical and transcriptional characteristics induced by various air humidity during desiccation of Norway spruce somatic embryos. The level of free polyamines lowered, higher forms of polyamines were favoured. Expression profiles of monitored genes were variously influenced by different relative air humidity. Our data proved, that desiccation of somatic embryos is metabolically active process highly affected by relative air humidity.