Štítky: somatická embryogeneze

Desiccation as a post-maturation treatment helps complete maturation of Norway spruce somatic embryos: carbohydrates, phytohormones and proteomic status

Napsal uživatel Kateřina Eliášová dne Po, 14.02.2022 - 11:30

Exposure of Norway spruce (Picea abies) somatic embryos and those of many other conifers to post-maturation desiccation treatment significantly improves their germination. An integration analysis was conducted to understand the underlying processes induced during the desiccation phase at the molecular level. Carbohydrate, protein and phytohormone assays associated with histological and proteomic studies were performed for the evaluation of markers and actors in this phase.

Assessment of autophagy in plant cells

Napsal uživatel Kateřina Eliášová dne Út, 21.04.2020 - 14:26

Autophagy is an evolutionary conserved catabolic process deemed to maintain or restore cellular and organismal homeostasis. In plants, basal autophagy is essential for growth and development, it is required for nutrient remobilization during senescence and nutrient deficiency, for removal of organelles and macromolecules formed during plant development or damaged by environmental stresses.

Cytological, Biochemical and Molecular Events of the Embryogenic State in Douglas-fir (Pseudotsuga menziesii [Mirb.])

Napsal uživatel Kateřina Eliášová dne St, 27.02.2019 - 17:19

Somatic embryogenesis techniques have been developed for most coniferous species, but only using very juvenile material. To extend the techniques’ scope, better integrated understanding of the key biological, physiological and molecular characteristics of embryogenic state is required. Therefore, embryonal masses (EMs) and non-embryogenic calli (NECs) have been compared during proliferation at multiple levels.

The response of Picea abies somatic embryos to UV-B radiation depends on the phase of maturation

Napsal uživatel Kateřina Eliášová dne Út, 27.11.2018 - 11:17

In summary, the exposure of Norway spruce EC to UV-B radiation at different stages of maturation had the strongest effect on cell viability of embryos at early stages of development and resulted in the inhibition of somatic embryo development and/or the substantial decrease in the number of embryos. The extent of cell damage was dependent on the UV-B dose applied, as well as the embryo developmental stage, and might be related to differentiation of the outermost cell layers and formation of protoderm.

Profiles of endogenous phytohormones over the course of Norway spruce somatic embryogenesis

Napsal uživatel Kateřina Eliášová dne Út, 21.08.2018 - 13:24

Conifer somatic embryogenesis (SE) is a process driven by exogenously supplied plant growth regulators (PGRs). Exogenous PGRs and endogenous phytohormones trigger particular ontogenetic events. Complex mechanisms involving a number of endogenous phytohormones control the differentiation of cells and tissues, as well as the establishment of structures and organs. Most of the mechanisms and hormonal functions in the SE of conifers have not yet been described.

Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii (Mirb.)

Napsal uživatel Kateřina Eliášová dne Út, 21.08.2018 - 11:54

This first report of cellular and molecular changes after repetitive somatic embryogenesis in conifers shows that each cycle enhanced the structure and singularization of EMs through modulation of growth regulator pathways, thereby improving the line´s embryogenic status.

Somatická embryogeneze smrku - proliferace nové embryogenní kultury

Napsal uživatel Kateřina Eliášová dne Pá, 05.03.2021 - 16:29

Nové buňky rostoucí na bývalém zygotickém embryu buď tvoří kalusy různých forem i barev nebo se diferencují na suspensorové a meristematické buňky raných somatických embryí a dávají základ nové embryogenní kultuře. 

Somatická embryogeneze smrku - indukce nových kultur

Napsal uživatel Kateřina Eliášová dne Pá, 05.03.2021 - 16:10

Nová somatická embryogenní kultura může vzniknout ze zygotického embrya kultivovaného in vitro na indukčním médiu . Důležitou roli v této fázi hrají fytohormony auxiny a cytokininy. 

Quantification of histochemical detection of polyphenolic compounds in somatic embryos of Norway spruce

Napsal uživatel Kateřina Eliášová dne St, 27.02.2019 - 16:54

Under standard cultivation conditions, Norway spruce somatic embryos (SEs) accumulate polyphenolic compounds mostly in the root cap. In response to UV-B, the accumulation of polyphenolics increases in protodermal cells and subprotodermal cortical cells and idioblasts in hypocotyl and cotyledons where they can attenuate UV-B stress. To quantify polyphenolic compounds accumulated in somatic embryos on the histological level resin sections were prepared, stained with Toluidine Blue.