Somatická embryogeneze smrku - indukce ze zygotických embryí
Somatickou embryogenní kulturu lze indukovat z nezralých zygotických embryí extirpovaných ze semen v průběhu léta.
Štítky: somatická embryogeneze
Quantification of histochemical detection of polyphenolic compounds in somatic embryos of Norway spruce
Under standard cultivation conditions, Norway spruce somatic embryos (SEs) accumulate polyphenolic compounds mostly in the root cap. In response to UV-B, the accumulation of polyphenolics increases in protodermal cells and subprotodermal cortical cells and idioblasts in hypocotyl and cotyledons where they can attenuate UV-B stress. To quantify polyphenolic compounds accumulated in somatic embryos on the histological level resin sections were prepared, stained with Toluidine Blue.
The effect of different air humidity during desiccation on the development of Norway spruce somatic embryos
The objective of the study was to follow morphological, selected biochemical and transcriptional characteristics induced by various air humidity during desiccation of Norway spruce somatic embryos. The level of free polyamines lowered, higher forms of polyamines were favoured. Expression profiles of monitored genes were variously influenced by different relative air humidity. Our data proved, that desiccation of somatic embryos is metabolically active process highly affected by relative air humidity.
The effect of drought stress on Norway spruce somatic embryo development
Somatic embryogenesis is a developmental process where a plant somatic cell dedifferentiate to a totipotent embryonic stem cell that has the ability to give rise to an embryo under appropriate conditions. Desiccation is the final phase of normal embryonic development in most angiosperms and appears to be important in the transition from embryogeny to the ability to germinate and form normal seedlings.
Somatická embryogeneze jehličnanů
Proces somatické embryogeneze rostlin umožňuje získat velké množství geneticky identických jedinců. V praxi se využívá k získání bohatého potomstva z minimálního množství semen. Zároveň je somatická embryogeneze ideálním systémem pro studium raných fází vývoje rostlin. Základním principem somatické embryogeneze je odvození embryogenní kultury většinou ze zygotických embryí a následně její kultivace in vitro na přesně definovaných médiích, jejichž složení určuje další vývoj somatických embryí.
What is the best way to maintain embryogenic capacity of embryogenic lines initiated from Douglas-fir immature embryos?
Douglas-fir (Pseudotsuga menziesii (Mirb) Franco) is a native conifer from the Pacific North-West of the US and Canada, and is one of the most important timber species used in the world. Douglas-fir is highly productive in a range of climatic conditions and has valuable wood properties (quality) as well as a strong tolerance to diseases and insects. In Europe, Douglas-fir is a major species for reforestation with increasing demand for its wood. Adaptation of new varieties to climate change and associated stresses is one challenging question for ongoing breeding programs.
Assessment of somaclonal variation in somatic embryo-derived plants of yacon [Smallanthus sonchifolius (Poepp. and Endl.) H. Robinson] using inter simple sequence repeat analysis and flow cytometry
Yacon (Smallanthus sonchifolius) is a root crop native to the Andean region. Low sexual reproductive capacity is a major constraint facing the genetic breeding of this crop. Biotechnological techniques offer alternative ways to widen genetic variability. We investigated somaclonal variation in regenerants of yacon derived from in vitro somatic embryogenesis using simple sequence repeat (ISSR) analysis and flow cytometry.
Early molecular events involved in Pinus pinaster Ait. somatic embryo development under reduced water availability: transcriptomic and proteomic analyses
Maritime pine somatic embryos (SEs) require a reduction in water availability (high gellan gum concentration in the maturation medium) to reach the cotyledonary stage. This key switch, reported specifically for pine species, is not yet well understood. To facilitate the use of somatic embryogenesis for mass propagation of conifers, we need a better understanding of embryo development.
Exogenous putrescine affects endogenous polyamine levels and the development of Picea abies somatic embryos
Vondráková Z, Eliášová K, Vágner M, Martincová O, Cvikrová M:
Exogenous putrescine affects endogenous polyamine levels and the development of Picea abies somatic embryos.
PLANT GROWTH REGULATION 75: 405-414, 2015.