In Norway spruce, as in many other conifers, the germination capacity of somatic embryos is strongly influenced by the desiccation phase inserted after maturation. The intensity of drying during desiccation eminently affected the formation of emblings (i.e., seedlings developed from somatic embryos). Compared to non-desiccated embryos, the germination capacity of embryos desiccated at 100% relative humidity was about three times higher, but the reduction of relative humidity to 95% and 90% had a negative effect on the subsequent embryo development.
Typy publikací: Journal
The transcriptomic (RNA-Sequencing) datasets collected in the course of floral induction in Chenopodium ficifolium 459
The transition from vegetative growth to reproduction is an essential commitment in plant life. It is triggered by environmental cues (day length, temperature, nutrients) and regulated by the very complex signaling gene network and by phytohormones. The control of flowering is well understood in Arabidopsis thaliana and in some crops, much less is known about the other angiosperms.
The high concentrations of abscisic, jasmonic, and salicylic acids produced under long days do not accelerate flowering in Chenopodium ficifolium 459
The survival and adaptation of angiosperms depend on the proper timing of flowering. The weedy species Chenopodium ficifolium serves as a useful diploid model for comparing the transition to flowering with the important tetraploid crop Chenopodium quinoa due to the close phylogenetic relationship. The detailed transcriptomic and hormonomic study of the floral induction was performed in the short-day accession C. ficifolium 459. The plants grew more rapidly under long days but flowered later than under short days.
Desiccation as a post-maturation treatment helps complete maturation of Norway spruce somatic embryos: carbohydrates, phytohormones and proteomic status
Exposure of Norway spruce (Picea abies) somatic embryos and those of many other conifers to post-maturation desiccation treatment significantly improves their germination. An integration analysis was conducted to understand the underlying processes induced during the desiccation phase at the molecular level. Carbohydrate, protein and phytohormone assays associated with histological and proteomic studies were performed for the evaluation of markers and actors in this phase.
Micropropagation of an ornamental shrub Disanthus cercidifolius Maxim. and using ISSR and flow cytometry
This study aimed to develop an efficient micropropagation protocol for Disanthus cercidifolius Maxim., an ornamental shrub. Sprouting buds of two genotypes (‘Trubaʼ and ‘PdSʼ) were used as an initial plant material.
The effect of storage conditions on the carotenoid and phenolic acid contents of selected apple cultivars
The present study investigated how different storage conditions affect antioxidant levels in three scab-resistant and powdery mildew-tolerant apple cultivars. The contents of carotenoids, along with free and glycosylated phenolic acids, were quantified in harvested apples and apples stored in boxes with controlled atmospheric conditions (1.2% O2 and 2.2% CO2) and temperature (1 °C), boxes with regulated temperature (1 °C) and storage rooms with a temperature between 1 and 4 °C.
Phenolic compounds play important roles in plant defences against both biotic and abiotic stressors, and many in crop plants are highly beneficial for human health, but their quantities and profiles are influenced by numerous factors. Thus, the purpose of this study was to quantify free and glycosylated phenolic acids in apples (important sources of beneficial phenolics) in the course of fruit development, at harvest and during 5 months of storage.
Chenopodium ficifolium flowers under long days without upregulation of FLOWERING LOCUS T (FT) homologs
Frequent duplications of the FLOWERING LOCUS T (FT) gene across various taxonomic lineages resulted in FT paralogs with floral repressor function, whereas others duplicates maintained their floral-promoting role. The FT gene has been confirmed as the inducer of photoperiodic flowering in most angiosperms analyzed to date.
Seed dormancy is an adaptive mechanism that allows seed germination under suitable environmental conditions. Germination of stored dormant seeds proceeds after dormancy breaking induced by stratification. To improve understanding of dormancy breaking in beechnuts, we: investigated effects of moisture content and temperature during storage; analysed contents of abscisic acid, abscisic acid metabolites and indole-3-acetic acid in embryonic axes during storage and stratification; and histochemically localized storage proteins.
Assessment of somaclonal variation in indirect morphogenesis-derived plants of Arracacia xanthorrhiza
The objective of this work was to induce and detect somaclonal variation in arracacha (Arracacia xanthorrhiza) plants regenerated via indirect morphogenesis, in order to evaluate the potential of this technique to produce new genotypes for breeding purposes of this crop.