Externí autoři: Petra Hlásná-Cepková | Laboratory of Biologically Active Compounds

Micropropagation of an ornamental shrub Disanthus cercidifolius Maxim. and using ISSR and flow cytometry

Lateral bud emerging from the primary explant of Disanthus cercidifolius

This study aimed to develop an efficient micropropagation protocol for Disanthus cercidifolius Maxim., an ornamental shrub. Sprouting buds of two genotypes (‘Trubaʼ and ‘PdSʼ) were used as an initial plant material.

Assessment of somaclonal variation in indirect morphogenesis-derived plants of Arracacia xanthorrhiza

Callus induction and plant regeneration of A. xanthorrhiza. (a) Callus with meristematic centres (bar = 3 mm) (b) Histological analysis of morphogenic callus (MC: Meristematic Centers) (bar = 200 µm ) (c) Shoots regeneration from callus (bar = 4 mm) (d) Germinated somatic embryo (bar = 1.2 mm) (e) Histological analysis of early cotyledonary somatic embryo (Ct: Cotyledon; Hy: Hypocotyl; Ra: Radicle; SA: Shoot Apex) (bar = 500 µm) (f) Plant recovered from callus (bar = 5 mm)

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The objective of this work was to induce and detect somaclonal variation in arracacha (Arracacia xanthorrhiza) plants regenerated via indirect morphogenesis, in order to evaluate the potential of this technique to produce new genotypes for breeding purposes of this crop.