Research

Annotation:

The aim of the project was to elucidate the role of proline and polyamines, metabolites with the crucial protective functions, in the stress response of tobacco plants to high temperature and drought.

Characterization of defence response in wild plants and in transgenic plants with modified P5CS gene (P5CSF129A), which codes for the key enzyme of proline biosynthesis, contributed to understanding of the function of these protective elements in plant abiotic stress tolerance.

Annotation:

The obtained results contributed to understanding of the role of polyamines and additional biological mechanisms in optimization of the induction of embryogenic lines and somatic embryo conversion of Hurst ecotype of Norway spruce. New knowledge on the genetic diversity of Hurst ecotype of Norway spruce will be used in reproduction the required genotypes.

Annotation:

We extend the information dealt with the concentration and composition of antioxidants in selected apple varieties from IEB. The research activity of the Station of apple breeding is focused on research of resistance against diseases in apples and on breeding of scab resistant and powdery mildew tolerant apple varieties. Breeding for resistance to these diseases has a high priority throughout the world in view of minimizing the use of chemicals in fruit production.

Biochemical and morphological parameters determinig cryotolerance in conifer embryogenic cultures.

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The aim of the project is to evaluate the relationship between polyamine metabolism and autophagy in plants. Autophagy will be followed in two experimental systems - in tobacco BY-2 cell culture and in coniferous embryogenic cultures. Firstly, the polyamine metabolism will be monitored during induced autophagy, then the process of autophagy will be studied after exogenous application of higher polyamines or endogenous modulation of polyamine synthesis. The obtained results will contribute to understanding of the function of polyamines in plant autophagy.

Role of ABSCISIC ACID.INSENSITIVE3 homologue in abscisic acid signal transduction studied in system of Norway spruce somatic embryogenesis.

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The aim of the COST Action CA15124 - A new Network of European BioImage Analysts to advance life science imaging (NEUBIAS) is to maximise the outputs of image-based research in life sciences by empowering the international network of bioimage analysis (IA) experts - BIAlysts.

Cultivation and genetic factors affecting efficiency of conifer somatic embryogenesis.

Annotation:

The aim of the project was to evaluate the alterations in polyamine metabolism and in the levels of hydroxycinnamic acids, flavonoids and stilbenes in the stress response of somatic embryos of Norway spruce to drought and high temperature. The determined contents of abscisic acid and auxin contributed to understanding the abscisic acid-auxin cross-talk in the response to stress. The extent of lipid peroxidation (as indicated by MDA levels) in stressed somatic embryos was also investigated. Histochemical tests for the presence of polyphenolic substances on embryo sections after the exposure of somatic embryos to UV-B completed the biochemical analyses.

Cultivation and genetic factors affecting efficiency of conifer somatic embryogenesis.

MŠMT, 2000-2004, Martin Vágner