Externí autoři: Lelu-Walter MA

What is the best way to maintain embryogenic capacity of embryogenic lines initiated from Douglas-fir immature embryos?

Submitted by Kateřina Eliášová on Tue, 03/27/2018 - 12:16

Douglas-fir (Pseudotsuga menziesii (Mirb) Franco) is a native conifer from the Pacific North-West of the US and Canada, and is one of the most important timber species used in the world. Douglas-fir is highly productive in a range of climatic conditions and has valuable wood properties (quality) as well as a strong tolerance to diseases and insects. In Europe, Douglas-fir is a major species for reforestation with increasing demand for its wood. Adaptation of new varieties to climate change and associated stresses is one challenging question for ongoing breeding programs.

Early molecular events involved in Pinus pinaster Ait. somatic embryo development under reduced water availability: transcriptomic and proteomic analyses

Submitted by Kateřina Eliášová on Tue, 02/13/2018 - 09:52

Maritime pine somatic embryos (SEs) require a reduction in water availability (high gellan gum concentration in the maturation medium) to reach the cotyledonary stage. This key switch, reported specifically for pine species, is not yet well understood. To facilitate the use of somatic embryogenesis for mass propagation of conifers, we need a better understanding of embryo development.

Unbiased estimation of the proportion of non-embryogenic cell clusters in the somatic embryogenic culture of Douglas-fir

Submitted by Kateřina Eliášová on Mon, 02/12/2018 - 23:26

Embryogenic cultures of Douglas-fir were induced from immature zygotic embryos. They proliferate as embryonal masses, however, in some lines of Douglas-fir, non-embryogenic cell (NECs) clusters interspersed with early somatic embryos (SEs) were observed. In order to evaluate the differences between lines we wanted to quantify the proportion of the SEs and NECs in the embryonal mass. For estimation of this proportion (volume density) we used stereological point-counting method based on counting points of the test grid falling in the tissue under study.