It is necessary to find convenient and measurable criteria for the evaluation of seeds/embryos quality before germination because of a long time interval between the preparation of seeds or embryos (before germination) and obtaining the seedlings or emblings. The success of all treatments before germination is assessed by the quality of the newly formed plants only.
Vaňková R, Kosová K, Dobrev P, Vítámvás P, Trávníčková A, Cvikrová M, Pešek B, Gaudinová A, Přerostová S, Musilová J, Galiba G, Prášil IT:
Dynamics of cold acclimation and complex phytohormone responses in Triticum monococcum lines G3116 and DV92 differing in vernalization and frost tolerance level.
Maritime pine somatic embryos (SEs) require a reduction in water availability (high gellan gum concentration in the maturation medium) to reach the cotyledonary stage. This key switch, reported specifically for pine species, is not yet well understood. To facilitate the use of somatic embryogenesis for mass propagation of conifers, we need a better understanding of embryo development.
Eliášová K, Pešek B, Vondráková Z:
Storage compounds, ABA and fumarase in Fagus sylvatica embryos during stratification.
DENDROBIOLOGY 74: 25-32, 2015.
Higher polyamine levels of fully developed embryos had positive effects on their ability to tolerate UV-B irradiation when compared with induced response s of early embryos. The aim of this work was to test the hypothesis that the higher levels of polyamines (PAs) might be involved in the response of Norway spruce somatic embryos to UV-B irradiation. We compare here the effects of 0.1, 0.6 and 6 W m-2 h-1 UV-B irradiation on polyamine metabolism in early and fully developed Norway spruce somatic embryos.
Key message The results of this study indicate the roles of polyamines and phenylpropanoids in the prevention of oxidative damage provoked by UV-B treatment. Abstract Plants respond to UV-B exposure by synthesizing a broad range of secondary metabolites, including ROSscavenging antioxidants and UV-B screening phenylpropanoids. Our recent results indicated that the accumulation of higher levels of polyamines in fully developed somatic embryos of Norway spruce may be causally linked to better tolerance of UV-B irradiation.
Somatic embryogenesis (SE) in conifers is governed by a complex network of hormonal metabolic and signaling pathways. Changes in the patterns and concentrations of endogenous phytohormones including auxins, cytokinins (CKs), abscisic acid (ABA), jasmonates, and salicylic acid (SA) were analyzed in the course of SE in Norway spruce (Picea abies).
