It is necessary to find convenient and measurable criteria for the evaluation of seeds/embryos quality before germination because of a long time interval between the preparation of seeds or embryos (before germination) and obtaining the seedlings or emblings. The success of all treatments before germination is assessed by the quality of the newly formed plants only.
Externí autoři: Pešek B
Dynamics of cold acclimation and complex phytohormone responses in Triticum monococcum lines G3116 and DV92 differing in vernalization and frost tolerance level
Vaňková R, Kosová K, Dobrev P, Vítámvás P, Trávníčková A, Cvikrová M, Pešek B, Gaudinová A, Přerostová S, Musilová J, Galiba G, Prášil IT:
Dynamics of cold acclimation and complex phytohormone responses in Triticum monococcum lines G3116 and DV92 differing in vernalization and frost tolerance level.
Early molecular events involved in Pinus pinaster Ait. somatic embryo development under reduced water availability: transcriptomic and proteomic analyses
Maritime pine somatic embryos (SEs) require a reduction in water availability (high gellan gum concentration in the maturation medium) to reach the cotyledonary stage. This key switch, reported specifically for pine species, is not yet well understood. To facilitate the use of somatic embryogenesis for mass propagation of conifers, we need a better understanding of embryo development.
Eliášová K, Pešek B, Vondráková Z:
Storage compounds, ABA and fumarase in Fagus sylvatica embryos during stratification.
DENDROBIOLOGY 74: 25-32, 2015.
The impact of UV-B irradiation applied at different phases of somatic embryo development in Norway spruce on polyamine metabolism
Higher polyamine levels of fully developed embryos had positive effects on their ability to tolerate UV-B irradiation when compared with induced response s of early embryos. The aim of this work was to test the hypothesis that the higher levels of polyamines (PAs) might be involved in the response of Norway spruce somatic embryos to UV-B irradiation. We compare here the effects of 0.1, 0.6 and 6 W m-2 h-1 UV-B irradiation on polyamine metabolism in early and fully developed Norway spruce somatic embryos.
Key message The results of this study indicate the roles of polyamines and phenylpropanoids in the prevention of oxidative damage provoked by UV-B treatment. Abstract Plants respond to UV-B exposure by synthesizing a broad range of secondary metabolites, including ROSscavenging antioxidants and UV-B screening phenylpropanoids. Our recent results indicated that the accumulation of higher levels of polyamines in fully developed somatic embryos of Norway spruce may be causally linked to better tolerance of UV-B irradiation.